THE ANTIBACTERIAL IMPACT OF EQUISETUM ARVENSE AND DETECTION OF ACTIVE COMPOUNDS

Abstract

Background: Methods: In this study, A different range of concentrations was applied (50, 25 and 12.5 %)w/v  of methanolic and aquatic extract of Equisetum arvense L. for their antibacterial activity against five types of bacteria (Staphylococcus spp, Pseudomonas aeruginosa, and Proteus spp and E. coli). The antibacterial activity has been measured using the zone of inhibition and also performed an antibiotic test using erythromycin E, ciprofloxacin CIP, ampicillin-sulbactam SAM, cefoxitin FOX, oxytetracycline T50, and trimethoprim-sulphamethoxazole TS. Results: The results showed that the methanolic extract had a broad-spectrum activity and was particularly effective against Staphylococcus spp (28 mm inhibition zone) and less effective against E. coli (27 mm inhibition zone). The aquatic extract had a weaker effect, with a 15 mm inhibition zone for Staphylococcus spp and a 14 mm inhibition zone for E. coli. Salmonella spp and Proteus spp were insensitive to the aquatic extract. Regarding antibiotics despite Pseudomonas aeruginosa being susceptible to all antibiotics, Salmonella spp was only resistant to all antibiotics except ciprofloxacin