DETECTION OF ANTI-SPERM ANTIBODIES AND COMPLEMENT 5A IN PRIMARY INFERTILITY MALES IN SALAH AL-DIN, IRAQ

Abstract

The objective of this study was to examine the immunological markers of anti-sperm antibody (ASA) and complement5a (C5a). The individuals seeking medical care are referred to Urology Department at Tikrit Teaching Hospital in Salah Al-Din Hospital (Iraq) and a private laboratory. A total of 110 patients and controls were included in the study, with recruitment taking place between May 2022 and June 2023. Samples of semen and blood were obtained from both infertile males and control subjects in order to conduct an immunological examination of seminal fluid. The control group comprised 50 individuals who were randomly selected from the population of Tikrit city during the study period. The patients were distributed into three groups: Azoospermic (AZO), Asthenozoospermia (AST) and Oligozoospermic (OLI) . Anti-sperm antibody (ASAs) and C5a levels were assessed in seminal plasma fluid and serum by Enzyme Linked Immuno Sorbant Assay (ELISA). The immunological investigation during present study, showed that among 60 samples (patients), there were 25 (41.6%) with positive anti-sperm antibody in their seminal fluids, while 35 (58.3%) patient with negative anti-sperm antibody in their seminal fluid plasma. Additionally, the study demonstrated a notable disparity in serum C5a levels between those with normospermia and those with oligozoospermia.